Journal article
Biochemical Characterization of Caenorhabditis elegans Ferritins
Sanjeedha SM Mubarak, Tess R Malcolm, Hamish G Brown, Eric Hanssen, Megan J Maher, Gawain McColl, Guy NL Jameson
Biochemistry | American Chemical Society | Published : 2023
Abstract
The nematode Caenorhabditis elegans contains genes for two types of ferritin (ftn-1 and ftn-2) that express FTN-1 and FTN-2. We have expressed and purified both proteins and characterized them by X-ray crystallography, cryo-electron microscopy, transmission electron microscopy, dynamic light scattering, and kinetically by oxygen electrode and UV-vis spectroscopy. Both show ferroxidase activity, but although they have identical ferroxidase active sites, FTN-2 is shown to react approximately 10 times faster than FTN-1, with L-type ferritin character over longer time periods. We hypothesize that the large variation in rate may be due to differences in the three- and four-fold channels into the ..
View full abstractRelated Projects (2)
Grants
Awarded by Australian Research Council (ARC)
Awarded by ARC Future Fellowship
Awarded by US National Institutes of Health__Office of Research Infrastructure Programs
Awarded by Australian Research Council
Funding Acknowledgements
This study was supported by grants from the Australian Research Council (ARC) to G.M. (DP180101248), to G.N.L.J. and G.M. (DP200100110), and from the Victorian Government's Operational Infrastructure Support Program. M.J.M. was supported by an ARC Future Fellowship (FT180100397). We thank the Caenorhabditis Genetics Center (CGC) supported by the US National Institutes of Health__Office of Research Infrastructure Programs (P40 OD010440) for providing C. elegans strains. Part of this study was carried out using the MX2 beamline at the Australian Synchrotron, which is part of ANSTO, and made use of the ACRF detector. We thank the beamline staff for their enthusiastic and professional support. TEM was carried out at the Bio21 Institute Ian Holmes Imaging Centre (University of Melbourne) and the ARC Centre for Cryo-Electron Microscopy of Membrane Proteins. We thank the Centre staff for their technical support in data collection and in image processing. We thank the Melbourne Mass Spectrometry and Proteomics Facility of The Bio21 Molecular Science and Biotechnology Institute at The University of Melbourne for the